产品货号：
GS2196
中文名称：
BstFNI内切酶
英文名称：
BstFNI
产品规格：
300U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
技术规格
相关搜索：BstFNI内切酶，BstFNI限制性内切酶
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA in 1hourat 60℃ in a total reaction volume of 50μl |
| Recognition sites | CG↑CG |
| 来源 | Bacillus stearothermophilus FN |
| Assayed on | Lambda DNA |
| Optimal buffer | Y(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 60℃ |
| Storage conditions | 20 mM Tris-HCl(pH 7.5);300 mM NaCl;0.1 mM EDTA;10 mM MgCl2;200 μg/ml BSA,7 mM 2-mercaptoethanol and 50%glycerol;Store at -20℃. |
| Ligations | After 5-fold overdigestion with enzyme more than 95%of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 10 u.a.of enzyme for 16hoursat 60℃. |
| Methlation effect | Blocked by CG methylation |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | AccII,Bsh1236I,BspFNI^,BstUI,FnuDII^,MvnI,ThaI |
相关搜索：BstFNI内切酶，BstFNI限制性内切酶